Fertilization, a key biological process in sexual reproduction, requires interaction of the male spermatozoon and the female oocyte. In most animals, spermatozoa move toward an ooctye to fertilize it. Thus spermatozoa motility is a key determinant of male fertility. In bivalves and fishes, similar to higher vertebrates, spermatozoa are immotile in the testis and sperm duct due to osmolality, potassium ions or pH. In fish, spermatozoa acquire motility potential during transit through sperm duct, and its motility is triggered after discharge into a hypo- or hyper-osmolality environment in freshwater and marine species, respectively (Figure 1). In bivalves, we reported that spermatozoa motility is not induced by environmental osmolality, and also the neurotransmitter serotonin is capable of hyperactivating spermatozoa motility (Figure 2). In most fishes, duration of spermatozoa motility is short, lasting for a few seconds to few minutes due to rapid depletion of energy required for the motility (Video 1). In bivalves, the duration of spermatozoa motility is longer, from minutes to hours. We are conducting further experiments to better understand evolutionary biology of sperm in aquatic animals with emphasize on morphology, energetics, motility signaling, and flagellar beating behavior and kinetics.

Figure 1. Our current knowledge on spermatozoa motility signaling in sturgeons. In a hypo-osmotic environment, a decrease in environmental K⁺ concentrations causes K⁺ efflux through unknown K⁺ channel(s). This hypothetically leads to a rapid membrane hyperpolarization, which in turn stimulates Ca²⁺ influx via Ca²⁺ channels. Contribution of [Ca²⁺]i stores to increase [Ca²⁺]i is unknown. However, rise in of [Ca²⁺]i induces Ca²⁺/calmodulin (CaM) activated phosphodiesterase, and also activates protein kinase C (PKC) localized at the midpiece and the flagellum. The PKC regulates activity of a dynein intermediate chain 2 (IC2). Both Ca²⁺/CaM proteins and IC2 are involved in axonemal beating. Demembranated spermatozoa of sturgeons require cAMP in the reactivation medium for the axonemal beating. The PKA has been recently shown to be localized in the flagella and head regions that regulate phosphorylation mitochondrial and cytoplasmic proteins that regulate homeostasis of energy and axonemal beating, respectively. Reference: Alavi, S.M.H., Cosson, J., Bondarenko, O., Linhart, O., 2019. Sperm motility in fishes: (III) diversity of regulatory signals from membrane to the axoneme. Theriogenology, September 15, 136: 143–165.

Figure 2. Current known signaling pathway of spermatozoa motility in bivalves. During spawning, spermatozoa motility is triggered by a Na+-dependent alkalization of internal pH mediated by a Na+/H+ exchanger. In addition, decrease of extracellular K+ or presence of extracellular 5-hydroxytryptamine creatinine sulfate (5-HT) induces hyperpolarization of spermatozoa membrane potential (Em), mediated by K+ efflux via voltage-dependent K+ channels and 5-HT influx, associated with opening of voltage-dependent Ca2+ channels under alkaline condition. Subsequent increase in intracellular Ca2+ content ([Ca2+]i) triggers Ca2+/calmodulin (CaM)-dependent flagellar beating. Release of Ca2+ from intracellular stores may also contribute to increase [Ca2+]i. Na+ influx probably regulates Ca2+ exchange via Na+/Ca2+ exchanger. The mechanism through which 5-HT induces spermatozoa hyper-motility is unclear and it may be possible that 5-HT is involved in controlling intracellular cAMP rise in bivalve spermatozoa, leading to cAMP-dependent protein kinase (PKA) phosphorylation in the flagellum. Reference: Boulais, M., Demoy-Schneider, M., Alavi, S.M.H., Cosson, J., 2019. Spermatozoa motility in bivalves: Signaling, flagellar beating behavior, and energetics. Theriogenology, September 15, 136: 15–27.

Environmental contaminants-related reproductive disorders

Public concern regarding endocrine-related reproductive disorders has increased due to increasing global rates of infertility. Aquatic environment throughout the world are repositories for enormous amounts of environmental contaminants (ECs) that may act as endocrine disrupting chemicals. According to World Health Organization, there are about 800 natural and man-made chemicals known or suspected to interfere with endocrine system. However a small fraction of ECs have been investigated to identify their effects on reproduction. To reduce exposure to ECs, and to evaluate associations between exposure and fertility, it is critical to understand how and when they act on reproductive system. However, the sites of action of ECs to reduce fertility are highly diverse. In males, ECs may reduce sperm production, cause damage to sperm morphology, alter sperm genome or decrease sperm motility and velocity to cause fertility threat (Figure 4). If ECs acts as EDCs, the adverse effects on reproductive system is associated with changes in hormonal functions of hypothalamus-pituitary-testis (Figure 5). Our lab strives to uncover the consequences of ECs on reproductive system and quality of gametes. We use fishes and rats as model organisms to elucidate a detailed risk assessment of ECs and to establish novel or more sensitive endpoints for ECs-related fertility threat.

Figure 4. The adverse effects of environmental contaminants (ECs) on fertility in male fishes. The aquatic environments are the final repository of the municipal wastewater, and industrial, agricultural and treatment plants effluents that contain various natural and man-made contaminants including metals, pesticides, pharmaceuticals and organic compounds as well as compounds used in personal care products. Evidences from wildlife-based and results of laboratory-based studies reveal adverse effects of ECs on sperm production, morphology, genome and motility to cause fertility threat at the level of individual. Contribution of ECs to decline fish population is largely unknown and needs to be elucidated. Reference: Alavi, S.M.H., Barzegar-Fallah, S., Rahdar, P., Ahmadi, M.M., Yavari, M., Hatef A., Golshan, M., Linhart, O., 2021. A review on environmental contaminants-related fertility threat in male fishes: Effects and possible mechanisms of action learned from wildlife and laboratory studies. Animals, October, 11(10): 2817.

 

Figure 5. A model representing neuroendocrine and endocrine disorders in goldfish exposed to di-(2-ethylhexyl)-phthalate (DEHP) that lead to diminished sperm quality. Following a chronic exposure (15–30 d), 11-KT levels were reduced in goldfish exposed to 1, 10 or 100 ug/L DEHP. At the same time, circulatory LH levels were decreased in DEHP treated goldfish. In addition, StAR mRNA levels were decreased in DEHP treated goldfish following 30 d of exposure. However, DEHP was without effects on gnrh3, kiss1, kiss2 and gpr54 mRNA levels. Overall, DEHP acts on pituitary and testicular hormonal functions to reduce sperm production, motility and velocity in goldfish. Reference: Golshan, M.,* Hatef, A.,* Socha, M., Milla, S., Butts, I.A.E., Carnevali, O., Rodina, M., Sokołowska-Mikołajczyk, M., Fontaine, P., Linhart, O., Alavi, S.M.H., 2015. Di-(2-ethylhexyl)-phthalate disrupts pituitary and testicular hormonal functions to reduce sperm quality in mature goldfish. Aquatic Toxicology, June, 163: 16–26. *Both authors contributed equally to this work.

Metabolic-related reproductive disorders

Reproductive function is highly sensitive to changes in the metabolic status and energy reserves of an organism. This is due to fact that several metabolic peptides/hormones are involved in regulation of hormonal functions of hypothalamus-pituitary-gonad. It has shown that adverse alternations in parental metabolic functions are associated with reproductive disorders that may critically affect their quality of gametes and fertility (Figure 3). The metabolic-related reproductive disorders in parents may result in epigenetic transgenerational inheritance of reproductive disease in subsequent generations. Currently, obesity, diabetes mellitus, use of performance-enhancing drugs, and poverty-related food deprivation are among very important global health concerns. We use rat and fish as model organism to identify metabolic-related reproductive disorders, to elucidate cell and molecular mechanisms through which metabolic alternations cause reproductive dysfunction that my result in fertility threat, and to investigate metabolic-related epigenetic transgenerational inheritance of reproductive diseases.

 

Figure 3. Gonadosomatic index (GSI) changes following administration of anabolic–androgenic steroids (AAS). Eight-weeks old male rates were intramusculary treated with 1, 2 or 9 mg/kg body weight per week nandrolone decanoate (ND) and 1 and 2 mg/kg body weight per week testosterone enanthate (TE). Following 4 weeks (top) and 8 weeks (bottom) of AAS, the testes were samples and GSI was measured as weight of testes / body mass x 100. Values are expressed as mean ± SEM. Reference: Karimi, S., 2021. Androgenic-anabolic steroids-disrupted endocrine regulation of spermatogenesis in rats. M.Sc. thesis, School of Biology, College of Science, University of Tehran, Tehran.

 

 

 

Sayyed Mohammad Hadi Alavi		PhD	Cell and Molecular Biology of Reproduction
Mahboubeh Mahlouji		D4	Reproductive disorders in male fish exposed to crude oil pollutions: Mechanisms of action of polycyclic aromatic hydrocarbons on sperm production and maturation
Negar Kazori		D3	Androgen regulation of kisspeptin-GnRH system on gonadotropins release from pituitary
Asrasadat Seyednejad		M2	TBD
Maryam Darjezani		M2	The effects of food deprivation on metabolism of the thyroid hormones and steroidogenic cytochrome P450 expression in goldfish (Carassius auratus)
Parmoun Nekounam		M2	Expression of aryl hydrocarbon receptors and steroidogenic cytochrome P450 enzymes in goldfish (Carassius auratus) exposed to crude oil
Roghayeh Gheybi		M2​​​​​​​	Reproductive disorders in the female Donkey croaker (Pennahia aneus) sampled from the Mousa bay with crude oil pollution
Hadi Shahmoradi		M2	TBD
Vahidehsadat Mirjalili		Visiting student
Sotoodehsadat Alavi	​​​​​​​	Visiting student

 

 

 

 

 

 

 

Former members (*, co-supervision)

	Degree	Graduated	Thesis / dissertation
Vahidehsadat Mirjalili	MSc	Sep 16, 2024	Hormonal alternations of hypothalamus–pituitary–ovary axis and oocyte quality in female goldfish (Carassius auratus) under food deprivation
Negar Kazori	MSc	Sep 13, 2022	Transgenerational effects of nandrolone decanoate misuse on reproductive system and fertility in male F1 progeny of rat
Fatemeh Zahra Tondrow	MSc	Sep 13, 2022	Anthropological features of infertility and sexual dysfunction in patients with diabetes mellitus
Sargol Asadi	MSc	Sep 15, 2022	Influence of bacterial contamination of Vibrio harveyi and Pseudomonas aeruginosa and the effect of different concentrations of gentamicin on Zebrafish (Danio rerio) spermatozoa quality during short-term storage
Sara Alijanpour	MSc	Sep 12, 2021	Metabolic stress and hormonal functions of the thyroid gland in animal models
Shole Karimi	MSc	Sep 12, 2021	Anabolic-androgenic steroids-disrupted endocrine regulation of spermatogenesis in rats
Saba Faghihzadeh	MSc	Sep 27, 2021	The effectsof diazinon on thyroxine metabolismin goldfish (Carassius auratus)
Somayeh Hashemi	MSc	Sep 20, 2021	The effect of organophosphate insecticide diazinon on hormonal functions of the thyroid gland in goldfish (Carassius auratus)
Mohammad Alim Sediqi	MSc	Sep 22, 2019	Effects of temperature on sperm production and motility in goldfish (Carassius auratus)
Mahdi Golshan*	PhD	Sep 15, 2015	Environmental contaminants and endocrine associated male infertility in fish
Azadeh Hatef*	PhD	Sep 12, 2012	Sperm functions impairments and steroidogenesis transcriptomic alternations in fish exposed to endocrine disrupting chemicals

 

Please follow our publications at:

https://scholar.google.co.jp/citations?user=tnT8O0MAAAAJ&hl=en

 

Book and Special Issues

 

 

5. Alavi, S.M.H., Butts, I.A.E., 2024. Reproduction and Development in Aquatic Animals. In progress, Heliyon, ISSN 2405-8440, Elsevier.

4. Kowalski, R., Alavi, S.M.H., Dzyuba, B., Żarski, D., Horváth, Á., 2021. Reproductive Biology in Aquatic Animals: Implications for Aquaculture and Environmental Conservation. Animals, ISSN 2076-2615, Multidisciplinary Digital Publishing Institute (MDPI).

3. Alavi, S.M.H., Cosson, J., 2019. Reproductive Biology in Male Aquatic Animals: From Stem Cells to Sperm – a review collection. Theriogenology, ISSN 0093-691X, Elsevier.

2. Alavi, S.M.H., Linhart, O., Rosenthal, H., 2008. The Proceedings of the 1^st International Workshop on the Biology of Fish Sperm. Journal of Applied Ichthyology, Volume 24, Issue  4, ISSN 0175-8659, Wiley-Blackwell.

1. Alavi, S.M.H., Cosson, J.J., Coward, K., Rafiee, G., 2008. Fish Spermatology. Alpha Science Ltd, Oxford, UK, ISBN 978-1-84265-369-2.

 

 

Book Chapters and Review Articles

18. Alavi, S.M.H., Barzegar-Fallah, S., Butts, I.A.E., Osada, M., 2024. Physiological regulation of oocyte maturation in bivalves – a review. Under preparation.

17. Alavi, S.M.H., 2024. Sperm biology and control of reproduction in zebrafish (Danio rerio) – a review. Under preparation.

16. Shazada, N.E., Alavi, S.M.H., Siddique, M.A.M., Cheng, Y., Zhang, S., Rodina, M., Kocour, M., Linhart, O., 2024. Short-term storage of sperm in common carp from laboratory research to commercial production – A review. Reviews in Aquaculture, January, 16(1): 174–189. Doi: 10.1111/raq.12827

15. Zhang, S., Cheng, Y., Věchtová, P., Boryshpolets, S., Shazada, N. E., Alavi, S.M.H., Cosson, J., Linhart, O., 2023. Potential implications of sperm DNA methylation functional properties in aquaculture management. Reviews in Aquaculture, March, 15(2): 536–556. Doi: 10.1111/raq.12735

14. Rafiee, G., Alavi, S.M.H., Sperm morphology and quality. In: Fundations of Marine Fish Reproduction and Culture, Rafiee, G. (Ed.). The University of Tehran Press, Tehran, Iran, pp. 273–293. ISBN: 978-964-03-0362-7

13. Alavi, S.M.H., Barzegar-Fallah, S., Rahdar, P., Ahmadi, M.M., Yavari, M., Hatef, A., Golshan, M., Linhart, O., 2021. A review on environmental contaminants-related fertility threat in male fishes: Effects and possible mechanisms of action learned from wildlife and laboratory studies. Animals, October, 11: 2817. Doi: 10.3390/ani11102817

12. Alavi, S.M.H., 2020. A review on sperm cryobanking in sturgeons. In: Collection of specialized meeting lectures on biodiversity and aquatic genetic resources, Golshan, M., Aghaei, M.J., Pourkazemi, M., Gaffari, H., Jorfi, E., Owfi, F., Yarmohammadi, M., Alavi, S.M.H., (Authors). Iranian Fisheries Science Research Institute, Registration No. 58315, Tehran, Iran, pp. 43–49. (In Farsi)

11. Alavi, S.M.H., Cosson, J., Bondarenko, O., Linhart, O., 2019. Sperm motility in fishes: (III) diversity of regulatory signals from membrane to the axoneme. Theriogenology, September 15, 136: 143–165. Doi: 10.1016/j.theriogenology.2019.06.038

10. Alavi, S.M.H., Nagasawa, K., Takahashi, K.G., Osada, M., 2017. Structure-function of serotonin in bivalve molluscs. In: Serotonin - A Chemical Messenger Between All Types of Living Cells, Kaneez, F.S., (Ed.). InTech - open science, pp. 33–63. ISBN: 978-953-51-3362-9. Doi: 10.5772/intechopen.69165

9. Alavi, S.M.H., Nagasawa, K., Takahashi, K.G., Osada, M., 2017. Pharmacology and molecular identity of serotonin receptor in bivalve mollusks. In: Serotonin - A Chemical Messenger Between All Types of Living Cells, Kaneez, F.S., (Ed.). InTech - open science, pp. 7–31. ISBN: 978-953-51-3362-9. Doi: 10.5772/intechopen.69680

8. Alavi, S.M.H., Ciereszko, A., Hatef, A., Křišťan, J., Dzyuba, B., Boryshpolets, S., Rodina, M., Cosson, J., Linhart, O., 2015. Sperm morphology, physiology, motility and cryopreservation in Percidae. In: Biology and Culture of Percid Fishes-Principles and Practices, Kestemont, P., Dabrowski, K., Summerfelt, R.C. (Eds.). Springer, Dordrecht, the Netherlands, pp. 163–191. ISBN: 978-94-017-7226-6. Doi: 10.1007/978-94-017-7227-3_5

7. Hatef, A., Alavi, S.M.H., Golshan, M., Linhart, O., 2013. Toxicity of environmental contaminants to fish spermatozoa functions in vitro – A review. Aquatic Toxicology, September 15, 140–141: 134–144. Doi: 10.1016/j.aquatox.2013.05.016

6. Alavi, S.M.H., Psenicka, M., Li, P., Hatef, A., Hulak, M., Rodina, M., Gela, D., Linhart, O., 2012. Sperm morphology and biology in sturgeon. In: Sperm Cell Research in the 21st Century: Historical Discoveries to New Horizens, Morisawa, M. (Ed.). Adthree Publishing Co., Ltd., Tokyo, Japan, pp. 100–112. ISBN: 978-4-904419-37-3

5. Alavi, S.M.H., Hatef, A., Pšenička, M., Kašpar, V., Boryshpolets, S., Dzyuba, B., Cosson, J., Bondarenko, V., Rodina, M., Gela, D., Linhart, O., 2012. Sperm biology and control of reproduction in sturgeon: (II) Sperm morphology, acrosome reaction, motility and cryopreservation. Reviews in Fish Biology and Fisheries, December, 22(4): 861–886. Doi: 10.1007/s11160-012-9270-x

4. Alavi, S.M.H., Rodina, M., Gela, D., Linhart, O., 2012. Sperm biology and control of reproduction in sturgeon: (I) Testicular development, sperm maturation and seminal plasma characteristics. Reviews in Fish Biology and Fisheries, September, 22(3): 695–717.  Doi: 10.1007/s11160-012-9268-4

3. Alavi, S.M.H., Linhart, O., Coward, K., Rodina, M., 2008. Fish spermatology: Implication for aquaculture management. In: Fish Spermatology, Alavi, S.M.H., Cosson, J.J., Coward, K., Rafiee, R. (Eds.). Alpha Science Ltd, Oxford, UK, pp. 397–460. ISBN: 978-1-84265-369-2

2. Alavi, S.M.H., Cosson, J., 2006. Sperm motility in fishes: (II) Effects of ions and osmotic pressure. Cell Biology International, January, 30(1): 1–14. Doi: 10.1016/j.cellbi.2005.06.004

1. Alavi, S.M.H., Cosson, J., 2005. Sperm motility in fishes: I. Effects of pH and temperature. Cell Biology International, February, 29(2): 101–110. Doi: 10.1016/j.cellbi.2004.11.021

 

 

Research Articles

94. Tondrow, F.Z., Alavi, S.M.H., Moradi, N., Gonadotropins, sex steroids and testicular histology in the Streptozotocin-induced diabetic models of male Wistar rats.

93. Kazori, N., Zeynali, B., Arefian, E., Alavi, S.M.H., Multigenerational effects of nandrolone decanoate abuse on reproductive system in male rats.

92. Alijanpour, S., Alavi, S.M.H., Kazori, N., Seddiqi, M.A.A., Circulating thyroid hormones levels in goldfish under absolute food deprivation.

91. Tondrow, F.Z., Alavi, S.M.H., Moradi, N., 2024. Hormonal functions of hypothalamus-pituitary-testis in Iranian patients with diabetes mellitus. Under preparation

90. Tondrow, F.Z., Barzegar-Fallah, S., Alavi, S.M.H., Moradi, N., 2024. Diabetic-disrupted hormonal functions of hypothalamus-pituitary-testis in men: A systematic review and meta-analysis. Under preparation

89. Mahlouji, M., Alavi, S.M.H., Kazori, N., Torfi, M., Jalili, A.H., Ghasemi, J., 2024. Gonadal histology and circulating sex steroids in Donkey croaker (Pennahia anea) inhabiting the Mousa Bay in the Persian Gulf with crude oil contamination. Under preparation

88. Kazori, N., Cheng, Y., Wang, J., Alavi, S.M.H., 2024. Anabolic-androgenic steroids-induced disruption of reproductive system in mammalian animal models: A systematic review and meta-analysis. Under preparation.

87. Cheng, Y., Nayak, R., Alavi, S.M.H., Linhartová, Z., Zhang, S., Waghmare, S.G., Rahi, D., Věchtová, P., Ma, Z., Labbe, C., Linhart, O., 2025. Comprehensive multi-omics analysis uncovers potential risks of sperm after short-term storage on offspring development

86. Mahlouji, M., Alavi, S.M.H., Ghasemi, J., Jalili, A.H., Torfi, M., Zhang, S., Shazada, N.E., Butts, I.A., Hoseinifar, S.H., Linhart, O., 2025. Crude oil–induced reproductive disorders in male goldfish: Testicular histopathology, sex steroids, and sperm swimming kinetics. Submitted.

85. Akbari Nargesi, E., Gorouhi, D., Alavi, S.M.H., Falahatkar, B., 2025. Spermiation induction in the common bream (Abramis brama) using sGnRHa and domperidone (Ovaprim): Sperm production, seminal plasma composition, and spermatozoa motility and fertilizing ability. Submitted.

84. Alavi, S.M.H., Barzegar-Fallah, S., Yoshida, M., Butts, I.A.E., Osada, M., 2025. Serotonin–induced sperm hyper-motility in Pacific oyster (Crassostrea gigas) associates with K+ efflux and membrane hyperpolarization. Submitted.

80 > 83. Cheng, Y., Wang, J., Alavi, S.M.H., Zhang, S., Linhartová, Z., Roy, D.R., Shazada, N.E., Dzyuba, B., Linhart, O., 2025. Impacts of global warming on quality of male fishes: evidence from meta-analysis of temperature effects on spermatozoa motility kinetics. Reviews in Aquaculture, Doi: 10.1111/raq.12961

82. Zhang, S., Waghmare, S.G., Alavi, S.M.H., Shazada, N.E., Cheng, Y., Muthu, K.E.S., Vechtová, P., Brionne, A., Nayak, R., Ma, Z., Labbé, C., Linhart, O., Linhartová, Z., 2025.  High temperature induces motility of zebrafish sperm after short-term storage. Aquaculture, January, 595: 741623. Doi: 10.1016/j.aquaculture.2024.741623

81. Asadi, S., Alavi, S.M.H., Moghimi, H., Zhang, S., Cheng, Y., Linhart, O., 2025. Gentamicin preserves motility and viability of Zebrafish (Danio rerio) sperm inoculated with bacteria during short-term storage. Iranian Journal of Fisheries Sciences, 24(1), Accepted.

80. Mirjalili, V., Alavi, S.M.H., 2024. Histology of digestive tract and liver in goldfish (Carassius auratus). Journal of Fisheries (University of Tehran Press), 77(4): Doi: 10.22059/jfisheries.2024.378706.1434 (In Farsi with English abstract).

79. Karimi, S.,* Kazori, N.,* Alavi, S.M.H., Alijanpour, S., Seddiqi, M.A.A., Zeynali, B., 2024. Nandrolone decanoate–induced hypogonadism in male rats: Dose- and time-dependent effects on pituitary and testicular hormonal functions. Physiological Reports, October, 12(19): e70053. Doi: 10.14814/phy2.70053. * Both authors equally contributed to the present study as the first author.

78. Xue, L., Gao, Y., Zhang, S., Weng, M., Zeng, G., Chen, J., Liao, M., Alavi, S.M.H., Guiguen, Y., 2024. Expression pattern of the fused in sarcoma gene and its contextual influence on the density-specific response of the growth hormone/insulin-like growth factor 1 axis in zig-zag eels (Mastacembelus armatus). Frontiers in Marine Science, September, 11: 1461451, Doi: 10.3389/fmars.2024.1461451

77. Shazada, N.E., Zhang, S., Siddique, M.A.M., Cheng, Y., Rodina, M., Alavi, S.M.H., Linhart, O., 2024. Optimizing extenders for short-term storage of Sterlet (Acipenser ruthenus) sperm. Aquaculture Reports, August, 37: 102216. Doi: 10.1016/j.aqrep.2024.102216  

76. Golshan, M.,* Alavi, S.M.H.,* Hatef, A., Kazori, N., Socha, M., Milla, S., Sokołowska-Mikołajczyk, M., Unniappan, S., Butts, I.A.E., Linhart, O., 2024. Impact of absolute food deprivation on the reproductive system in male goldfish exposed to sex steroids. Journal of Comparative Physiology Part B Biochemical, Systems and Environmental Physiology, August, 194(4): 411–426. Doi: 10.1007/s00360-024-01570-4, * Both authors equally contributed to the present study as the first author.

75. Cheng, Y., Zhang, S., Nayak, R., Vechtová, P., Schumacher, F., Linhartová, P., Linhartová, Z., Waghmare, S., Kleuser, B., Rodinová, V., Rodina, M., Sterba, J., Alavi, S.M.H., Labbé, C., Linhart, O., 2024. Fertilization by short-term stored sperm alters DNA methylation patterns at single-base resolution in common carp (Cyprinus carpio) embryos. Reviews in Fish Biology and Fisheries, September 34(3): 1167–1187. Doi: 10.1007/s11160-024-09866-y

74. Zhang, S., Bondarenko, O., Shazada, N.E., Cheng, Y., Rahi, D., Rodina, M., Gazo, I., Linhartová, Z., Alavi, S.M.H., Siddique, M.A.M., Boryshpolets, S., Dzyuba, B., Linhart, O., 2024. The plasma membrane contributes to temperature-induced spermatozoa motility kinetics in short-term stored sperm of Common carp (Cyprinus carpio). Aquaculture, March 30, 583: 740588. Doi: 10.1016/j.aquaculture.2024.740588

73. Cheng, Y., Waghmare, S., Zhang, S., Vechtová, P., Schumacher, F., Samarin, A.M., Samarin, A.M., Linhartová, Z., Dey, A., Brionne, A., Dietrich, M., Sterba, J., Alavi, S.M.H., Labbé, C., Linhart, O., 2023. Aging of common carp (Cyprinus carpio L.) sperm induced by short-term storage does not alter global DNA methylation and specific histone modifications in offspring. Aquaculture, June 30, 571(2023): 739484. Doi: 10.1016/j.aquaculture.2023.739484

72. Linhart, O., Cheng, Y., Zhang, S., Alavi, S.M.H., Rodinová, V., Shazada, N.E., Linhartová, Z., 2023. Sperm and egg in vitro storage effects on artificial fertilization and hatching in common carp (Cyprinus carpio L.). Aquaculture Reports, April, 29: 101507. Doi: 10.1016/j.aqrep.2023.101507

71. Zhang, S., Cheng, Y., Alavi, S.M.H., Shazada, N.E., Linhartová, Z., Rodinová, V., Linhart, O., 2023. Elevated temperature promotes spermatozoa motility kinetics and fertilizing ability following short-term storage: An implication for artificial reproduction of common carp Cyprinus carpio in a hatchery. Aquaculture, February 25, 565: 739126. Do: 10.1016/j.aquaculture.2022.739126

70. Khademzade, O., Kochanian, P., Zakeri, M., Alavi, S.M.H., Torfi Mozanzadeh, M., 2022. Oxidative stress-related semen quality and fertility in the male Arabian yellowfin sea bream (Acanthopagrus arabicus) fed a selenium nanoparticle-supplemented plant protein-rich diet. Aquaculture Nutrition. July, 2022: 3979203. Doi: 10.1155/2022/3979203

69. Golshan, M., Hatef, A., Kazori, N., Socha, M., Sokołowska-Mikołajczyk, M., Habibi, H.R., Linhart, O., Alavi, S.M.H., 2022. A chronic exposure to bisphenol A reduces sperm quality in goldfish associated with increases in kiss2, gpr54 and gnrh3 mRNA and circulatory LH levels at environmentally relevant concentrations. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology, July, 257: 109342. Doi: 10.1016/j.cbpc.2022.109342

68. Alavi, S.M.H., Hatef, A., Butts, I.A.E., Bondarenko, O., Cosson, J., Babiak, I., 2021. Some recent data on sperm morphology and motility kinetics in Atlantic cod (Gadus morhua L.). Fish Physiology and Biochemistry, April, 47(2): 327–338. Doi: 10.1007/s10695-020-00915-4

67. Nichols, Z.G., Rikard, S., Alavi, S.M.H., Walton, W.C., Butts, I.A.E., 2021. Regulation of sperm motility in Eastern oyster (Crassostrea virginica) spawning naturally in seawater with low salinity. PLoS ONE, March, 16(3): e0243569. Doi: 10.1371/journal.pone.0243569

66. Golshan, M., Hatef, A., Habibi, H.R., Alavi, S.M.H., 2020. A rapid approach to assess estrogenic transcriptional activity of bisphenol A in the liver of goldfish. Iranian Journal of Fisheries Sciences, 19(6): 2877–2892. Doi: 10.22092/IJFS.2020.122798

65. Golshan, M., Alavi, S.M.H., 2019. Androgen signaling in male fishes: Examples of anti-androgenic chemicals that cause reproductive disorders. Theriogenology, November, 139: 58–71. Doi: 10.1016/j.theriogenology.2019.07.020

64. Boulais, M., Demoy-Schneider, M., Alavi, S.M.H., Cosson, J., 2019. Spermatozoa motility in bivalves: Signaling, flagellar beating behavior, and energetics. Theriogenology, September 15, 136: 15–27. Doi: 10.1016/j.theriogenology.2019.06.025

63. Rajeswari, J.J., Hatef, A., Golshan, M., Alavi, S.M.H., Unniappan, S., 2019. Metabolic stress leads to divergent changes in the ghrelinergic system in goldfish (Carassius auratus) gonads. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, September, 235: 112–120. Doi: 10.1016/j.cbpa.2019.05.027

 

 

Other Publications: Forewords, Dedications and Methods

9. Alavi, S.M.H., 2021. Reproduction and infertility in men: A relationship between poverty and total fertility rate. Iranian Journal of Biology, 5(5), 60-69 (In Persian).

8. Alavi, S.M.H., Cosson, J., 2019. Reproductive biology in male aquatic animals: From stem cells to sperm – Foreword. Theriogenology, October 15, 138: 152-153.

7. Alavi, S.M.H., Cosson, J., 2019. The special issue "Reproductive biology in male aquatic animals: From stem cells to sperm" is dedicated to Professor Masaaki MORISAWA. Theriogenology, September 15, 136: 7–9.

6. Cosson, J., Linhart, O., Alavi, S.M.H., 2019. Obituary: Remembering Professor Roland Billard. Theriogenology, July 15, 133: 159–160.

5. Policar, T., Alavi, S.M.H., Stejskal, V., Křišťan, J., Kouřil, J., 2011. Umělý a poloumělý výtěr okouna říčního (Perca fluviatilis) používaný k masové produkci embryí. Edice Metodik (technologická řada), FROV JU Vodňany, 2011, č. 117, 34 s.

4. Alavi, S.M.H., Rodina, M., Gela, D., Linhart, O., 2009. Morfologie spermií, složení seminální plazmy, motilita a parametry zvlnění bičíku spermie u štiky obecné (Esox lucius). Bulletin VÚRH Vodňany 45(1): 3-9.

3. Policar, T., Stejskal, V., Bláha, M., Alavi, S.M.H., Kouřil, J., 2009. Technologie intenzivního chovu okouna říčního (Perca fluviatilis L.). Edice Metodik (technologická řada), FROV JU Vodňany, č. 89, 51 s.

2. Policar, T., Drozd, B., Kouřil, J., Kozák, P., Hamáčková, J., Alavi, S.M.H., Vavrečka, A., 2009. Současný stav, umělá reprodukce a odchov násadového materiálu parmy obecné (Barbus barbus L.). FROV JU Vodňany, Edice Metodik (technologická řada), č. 95, 39 s.

1. Policar, T., Toner, D., Alavi, S.M.H., Linhart, O., 2008. Reproduction and Spawning. In: Farming of Eurasian Perch Volume 1. Juvenile production, Rougeot, C., Torner, D., (Eds.). Special publication BIM, No. 24, 22-29.

 

 

Abstracts in Conferences, Symposiums and Workshops

84. Alavi, S.M.H., Kazori, N., Mirjalili, V., Tondrow, F., Mahlouji, M., Alijanpour, S., Siddiqi, M.A.A., 2024. Factors affecting reproductive system in fish: Nutrition and environmental contaminants. The 23th National & 11th International Congress on Biology, Tehran, Iran, September 9–11, 2024.

83. Kazori, N., Zeynali, B., Alavi, S.M.H., 2024. Modes of action of nandrolone decanoate to interfere with testosterone biosynthesis in male Wistar rats. Iran's 5^th Conference on Protein and Peptide Science, Tehran, Iran, May 8-9, 2024.

82. Mahlouji, M., Alavi, S.M.H., Ghasemi, J., Jalili, A.H., Torfi, M., Zhang, S., Shazada, N.E., Linhart, O., 2024. Exposure to crude oil decreases sperm motility in male goldfish. The 2^nd International Conference on Marine Sciences, Qeshm, Iran, February 27-28, 2024.

81. Mirjalili, V., Kazori, N., Golshan, M., Alavi, S.M.H., 2024. The hypothalamus–pituitary–ovary functions and oocyte quality in female goldfish under food deprivation. The 2^nd International Conference on Marine Sciences, Qeshm, Iran, February 27-28, 2024.

80. Kazori, N., Golshan, M., Hatef, A., Unniappan, S., Linhart, O., Alavi, S.M.H., 2024. Transcripts and localization of ghrelin and nesfatin-1 expressions in the testis of unfed goldfish. The 2^nd International Conference on Marine Sciences, Qeshm, Iran, February 27-28, 2024.

79. Alavi, S.M.H., Barzegar Fallah, S., Osada, M., 2022. Physiology of sperm motility initiation in bivalves. The 1^st International Conference on Marine Sciences, Bandar Abbas, Iran, December 17, 2022.

78. Khademzade, O., Alavi, S.M.H., Kochanian, P., Torfi Mozanzadeh, M., Zakeri, M., Shiri, N., 2022. Biology of sperm in the male Arabian yellowfin sea bream (Acanthopagrus arabicus). The 1^st International Conference on Marine Sciences, Bandar Abbas, Iran, December 17, 2022.

77. Kazori, N., Zeynali, B., Alavi, S.M.H., 2022. Intergenerational effects of nandrolone decanoate on reproductive system in male rats. The 22^th National & 10^th International Congress on Biology, Shahrekord, Iran, August 31–September 2, 2022.

76. Alavi, S.M.H., 2020. A state-of-the-art review of sperm motility signaling in fishes. The 19^th International Congress on Animal Reproduction. The 19^th International Congress on Animal Reproduction, Bologna, Italy, June 26–30, 2022.

75. Karimi, S., Rahdar, P., Alijanpour, S., Zeynali, B., Alavi, S.M.H., 2022. Anabolic-androgenic steroids inhibit testosterone and luteinizing hormone biosyntheses to cause hypogonadism in male rats. The 19^th International Congress on Animal Reproduction, Bologna, Italy, June 26–30, 2022.

74. Alavi, S.M.H., 2021. Regulatory mechanisms of sperm motility initiation in fishes - a review. The 21^th National & 9^th International Congress on Biology, Semnan, February 26-29, 2021, p. IBS2020-I9.

73. Alijanpour, S., Siddiqi, M.A., Alavi, S.M.H., 2021. Food deprivation decreases thyroxine conversion into triiodothyronine in goldfish.  The 21^th National & 9^th International Congress on Biology, Semnan, February 26-29, 2021, p. IBS2020-A2.

72. Nichols, Z.G., Rikard, S., Alavi, S.M.H., Bradford, J.A., Walton, W.C., Butts, I.A.E., 2020. Physiological mechanisms regulating sperm motility initiation in Eastern oyster, crassostrea virginica. World Aquaculture 2020. Singapore, June 8–12, 2020.

71. Alavi, S.M.H., Hatef, A., Butts, I.A.E., Cosson, J., Babiak, I., 2019. Sperm morphology and motility signaling in Atlantic cod, Gadus morhua.  The 7^th International Workshop on the Biology of Fish Gametes. Rennes, France, September 2–6, 2019, pp. 168–169.

70. Alavi, S.M.H., Golshan, M., Hatef, A., Socha, M., Milla, S., Sokołowska-Mikołajczyk M., Unniappan, S., Linhart, O., 2019. Metabolic stress decreases sperm quality in goldfish associated with changes in hormonal functions of hypothalamus-pituitary-testis axis. The 7^th International Workshop on the Biology of Fish Gametes. Rennes, France, September 2–6, 2019, pp. 36–37.

69. Alijanpour, S., Alavi, S.M.H., 2019. A systematic review of crosstalk between thyroid hormones and fertility in male fish. The 7^th International Workshop on the Biology of Fish Gametes. Rennes, France, September 2–6, 2019, pp. 91–92.

68. Chelengari, F., Alavi, S.M.H., Hatef, A., Velíšek, J., Rodina, M., Linhart, O., 2019. Stress reaction and sperm quality in hormonally treated sterlet (Acipenser ruthenus) for spermiation. The 7^th International Workshop on the Biology of Fish Gametes. Rennes, France, September 2–6, 2019, pp. 89–90.

67. Chelengari, F., Hatef, A., Velíšek, J., Rodina, M., Linhart, O., Alavi, S.M.H., 2019. Effects of stress induced by hormonal stimulation on sperm quality in sterlet (Acipenser ruthenus). The 3^rd Conference and 1^st National Congress on Fisheries Resources. Gorgan, Iran, April 30 – May 1, 2019.

66. Alavi, S.M.H., 2018. Modes of action of bis(2-ethylhexyl) phthalate, bisphenol A and vinclozolin on endocrine regulation of reproduction in male goldfish. International Conference on Society and Environment. Tehran, Iran, September 2, 2018.

65. Alavi, S.M.H., 2018. Sperm cryopreservation in sturgeons – A review. The Biological Conservation of Endangered Aquatic Organisms, Tehran, Iran, January 22, 2018.

Research Track - Web of Science (Sep, 20, 2023)

• Number of publications: 79
• H-Index: 30
• Total citations: 2623
• Citations without self-citations: 2280
• Citation per item: 33.2

 

 

Lab renovation. ©Photo: SMH Alavi	Lab renovation. ©Photo: SMH Alavi
Lab renovation. ©Photo: N Kazori	Lab renovation. ©Photo: N Kazori
Goldfish. ©Photo: SMH Alavi	Testicular histopathology in streptozotocin-induced diabetic male rats (B-G) compared to the control (A). ©Photo: F Tondrow
Aquarium system. ©Photo: N Kazori